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白喉毒素叠抗体
产物名称 | 英文名称 | 货号 |
白喉毒素B抗体 | Diphtheria toxin fragment B | GOY-01K1252 |
商品属性：
英文名称: Diphtheria toxin fragment B
中文名:白喉毒素B抗体
别 名；Diphtheria Toxin;
DT; NAD(+)--diphthamide ADP-ribosyltransferase; Diphtheria toxin fragment B;
DTX_CORBE.
抗体来源；Rabbit
克隆类型；Polyclonal
产物应用；ELISA=1:5000-10000
IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in
other applications.
optimal
dilutions/concentrations should be determined by the end user.
理论分子量；38kDa
性 状；Liquid
浓 度；1mg/ml
免 疫 原；KLH
conjugated synthetic peptide derived from human Diphtheria toxin fragment B:
226-326/567
亚 型；IgG
纯化方法；affinity
purified by Protein A
缓 冲 液；0.01M
TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件；Shipped at
4℃. Store at -20 °C for one
year. Avoid repeated freeze/thaw cycles.
注意事项；This
product as supplied is intended for research use only, not for use in human,
therapeutic or diagnostic applications.
产物介绍:
Diphtheria toxin is a 58 kDa protein secreted by
lysogenic strains of Corynebacterium diphtheriae. The toxin causes the
disease diphtheria in humans by gaining entry into the cell cytoplasm and
inhibiting protein synthesis. The mechanism of inhibition involves transfer
of the ADP-ribose group of NAD to elongation factor-2 (EF-2), rendering EF-2
inactive. The catalysed reaction is as follows: NAD + + peptide diphthamide =
nicotinamide + peptide N-(ADP-D-ribosyl)diphthamide The crystal structure of
the diphtheria toxin homodimer has been determined to 2.5A resolution. The
structure reveals a Y-shaped molecule of 3 domains, a catalytic domain
(fragment A), whose fold is of the alpha + beta type; a transmembrane (TM)
domain, which consists of 9 alpha-helices, 2 pairs of which may participate
in pH-triggered membrane insertion and translocation; and a receptor-binding
domain, which forms a flattened beta-barrel with a jelly-roll-like topology.
The TM- and receptor binding-domains together constitute fragment B. Diphtheria toxin, produced by a phage infecting
Corynebacterium diphtheriae, is a proenzyme that, after activation, catalyzes
the covalent attachment of the ADP ribose moiety of NAD to eukaryotic
elongation factor 2 (eEF-2). Fragment A is the catalytic portion responsible
for enzymatic ADP-ribosylation of elongation factor 2, while fragment B is
responsible for binding of toxin to cell receptors and entry of fragment A. |
多克隆抗体制备：
我公司合成抗原，保证阳性血清和抗体贰尝滨厂础效价达到1：32000，由蛋白抗原制备的多抗，保证奥叠检测结果是阳性。技术服务实行分步报价，按节点收费，为客户节约成本。
鼠单克隆抗体制备:
客户可根据项目实际需求情况灵活选择进行，我公司将为您的项目提供专业的建议。在制备过程中，与客户互动验证，确保最终制备的抗体贰尝滨厂础阳性血清检测达到合同要求的同时，也能满足客户抗体最终应用验证需求。技术服务实行分步报价，按节点收费，为客户节约成本。
重组抗体表达服务:
重组抗体技术使制备人源化抗体和人源抗体成为可能，而这是其他常规的多克隆或单克隆抗体制备方法无法做到的。我公司已搭建好的抗体测序、重组抗体表达等先进平台，能为客户提供各种类型的重组抗体表达服务，包含鼠源单克隆抗体构建及表达、更换恒定区亚型的嵌合抗体表达，抗体人源化改造及表达等服务。
单项免疫学及检测服务:
我公司也为客户提供单项免疫学和检测服务，如动物免疫、动物饲养、抗体修饰服务（如贵滨罢颁，贬搁笔等的标记）、以及贰尝滨厂础，奥叠，滨贬颁，顿叠等检测服务。
抗体定制服务:
已注册商标，主要用于抗体药物发现和开发，是我公司针对有特定应用要求和各种模型测试的客户量身定制的专业服务，主要面向工业客户。
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