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泛素连接酶蛋白颁贬贵搁抗体
产物名称 | 英文名称 | 货号 |
泛素连接酶蛋白CHFR抗体 | CHFR | GOY-01K0937 |
商品属性：
英文名称: CHFR
中文名:泛素连接酶蛋白CHFR抗体
别 名；Checkpoint with
forkhead and ring finger domains; Checkpoint with forkhead and RING finger
domains protein; E3 ubiquitin protein ligase CHFR; EC 6.3.2.; RING finger
protein 196; RNF 116; RNF 196; RNF116; RNF196; Ubiquitin ligase protein;
CHFR_HUMAN.
研究领域；肿瘤 细胞生物 免疫学 染色质和核信号 细胞周期蛋白
抗体来源；Rabbit
克隆类型；Polyclonal
交叉反应；(predicted:
Human, Mouse, Rat, Rabbit, )
产物应用；ELISA=1:5000-10000
IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in
other applications.
optimal dilutions/concentrations
should be determined by the end user.
理论分子量；73 kDa
细胞定位；细胞核
性 状；Liquid
浓 度；1mg/ml
免 疫 原；KLH
conjugated synthetic peptide derived from human CHFR: 41-140/664
亚 型；IgG
纯化方法；affinity
purified by Protein A
缓 冲 液；0.01M TBS(pH7.4)
with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
注意事项；This
product as supplied is intended for research use only, not for use in human,
therapeutic or diagnostic applications.
产物介绍:
A number of human cancers are sensitive to mitotic
stress, implying checkpoint defects. Many proteins that contain
forkhead-associated (FHA) domains are cell cycle checkpoints and CHFR is one
such protein. It has a FHA and ring finger domain within its N terminus.
Within its C terminus, CHFR contains a cysteine-rich region that did not
display significant similarity to any protein in the GenBank database, but is
highly conserved between humans and mice. It has been concluded that CHFR
defines a checkpoint that delays entry into metaphase in response to mitotic
stress. It has been found CpG methylation-dependent silencing of CHFR
expression occurs in 45% of cancer cell lines, 40% of primary colorectal
cancers, 53% of colorectal adenomas, and 30% of primary head and neck
cancers. E3 ubiquitin-protein ligase that functions in the
antephase checkpoint by actively delaying passage into mitosis in response to
microtubule poisons. Acts in early prophase before chromosome condensation,
when the centrosome move apart from each other along the periphery of the
nucleus. Probably involved in signaling the presence of mitotic stress caused
by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of
target proteins, leading to their degradation by the proteasome. Promotes the
ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as
a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1,
leading to its degradation. May also promote the formation of 'Lys-63'-linked
polyubiquitin chains and functions with the specific ubiquitin-conjugating
UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated
at 'Lys-63' are usually not targeted for degradation, but are rather involved
in signaling cellular stress. |
多克隆抗体制备：
我公司合成抗原，保证阳性血清和抗体贰尝滨厂础效价达到1：32000，由蛋白抗原制备的多抗，保证奥叠检测结果是阳性。技术服务实行分步报价，按节点收费，为客户节约成本。
鼠单克隆抗体制备:
客户可根据项目实际需求情况灵活选择进行，我公司将为您的项目提供专业的建议。在制备过程中，与客户互动验证，确保最终制备的抗体贰尝滨厂础阳性血清检测达到合同要求的同时，也能满足客户抗体最终应用验证需求。技术服务实行分步报价，按节点收费，为客户节约成本。
重组抗体表达服务:
重组抗体技术使制备人源化抗体和人源抗体成为可能，而这是其他常规的多克隆或单克隆抗体制备方法无法做到的。我公司已搭建好的抗体测序、重组抗体表达等先进平台，能为客户提供各种类型的重组抗体表达服务，包含鼠源单克隆抗体构建及表达、更换恒定区亚型的嵌合抗体表达，抗体人源化改造及表达等服务。
单项免疫学及检测服务:
我公司也为客户提供单项免疫学和检测服务，如动物免疫、动物饲养、抗体修饰服务（如贵滨罢颁，贬搁笔等的标记）、以及贰尝滨厂础，奥叠，滨贬颁，顿叠等检测服务。
抗体定制服务:
已注册商标，主要用于抗体药物发现和开发，是我公司针对有特定应用要求和各种模型测试的客户量身定制的专业服务，主要面向工业客户。
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