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腺苷受体蛋白抗体
产物名称 | 英文名称 | 货号 |
腺苷受体蛋白抗体 | cAMP-receptor protein | GOY-01K0246 |
商品属性：
英文名称: cAMP-receptor protein
中文名:腺苷受体蛋白抗体
别 名；CRP_ECOLI;
cAMP-activated global transcriptional regulator CRP; Catabolite activator
protein; CAP; Catabolite gene activator; cAMP receptor protein; CRP; cAMP
regulatory protein.
研究领域；细胞生物 免疫学 转录调节因子 细胞膜受体
抗体来源；Rabbit
克隆类型；Polyclonal
产物应用；WB=1:500-2000
ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in
other applications.
optimal
dilutions/concentrations should be determined by the end user.
理论分子量；24kDa
细胞定位；细胞膜
性 状；Liquid
浓 度；1mg/ml
免 疫 原；KLH
conjugated synthetic peptide derived from cAMP-receptor protein: 2-100/210
亚 型；IgG
纯化方法；affinity
purified by Protein A
缓 冲 液；0.01M
TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
注意事项；This
product as supplied is intended for research use only, not for use in human,
therapeutic or diagnostic applications.
产物介绍:
Escherichia coli contains more than 250 known
promoters that must be activated prior to transcription. cAMP receptor
protein (crp), also known as cAMP regulatory protein and catabolite gene
activator, is a 210 amino acid E. coli protein that complexes with cyclic AMP
and binds to specific DNA sites near the promoter, resulting in homodimeric
transcription activation of more than 100 E. coli promoters. Acting as a
negative regulator against its own synthesis, crp regulates transcription of
several catabolite-sensitive operons. Crp activates transcription by making
multiple interactions with RNA polymerase. Containing one cyclic nucleotide
binding domain and one HTH crp-type DNA binding domain, crp binds DNA as a
dimer to induce a severe bend in the DNA. This formation is a regulatory
mechanism of crp gene expression that plays a major role in determining the
molecular mechanism in gene transcription. A global transcription regulator. Complexes with
cyclic AMP (cAMP) which allosterically activates DNA binding (to consensus
sequence 5'-AAATGTGATCTAGATCACATTT-3') to directly regulate the transcription
of about 300 genes in about 200 operons and indirectly regulate the
expression of about half the genome. There are 3 classes of CRP promoters;
class I promoters have a single CRP-binding site upstream of the RNA
polymerase (RNAP)-binding site, whereas in class II promoters the single CRP-
and RNAP-binding site overlap, CRP making multiple contacts with RNAP. Class
III promoters require multiple activator molecules, including at least one
CRP dimer. It can act as an activator, repressor, coactivator or corepressor.
Induces a severe bend in DNA (about 87 degrees), bringing upstream promoter
elements into contact with RNAP. Acts as a negative regulator of its own
synthesis as well as for adenylate cyclase (cyaA), which generates cAMP. High
levels of active CRP are detrimental to growth (PubMed:16260780). Plays a
major role in carbon catabolite repression (CCR). CCR involves cAMP,
adenylate cyclase (cyaA), CRP and the EIIA-Glc component of the PTS (crr). In
the presence of glucose EIIA-Glc is dephosphorylated, and does not activate
adenylate cyclase, leading to reduced cAMP and thus decreased CRP activity.
Also plays a role in many other processes (see PubMed:22573269). |
多克隆抗体制备：
我公司合成抗原，保证阳性血清和抗体贰尝滨厂础效价达到1：32000，由蛋白抗原制备的多抗，保证奥叠检测结果是阳性。技术服务实行分步报价，按节点收费，为客户节约成本。
鼠单克隆抗体制备:
客户可根据项目实际需求情况灵活选择进行，我公司将为您的项目提供专业的建议。在制备过程中，与客户互动验证，确保最终制备的抗体贰尝滨厂础阳性血清检测达到合同要求的同时，也能满足客户抗体最终应用验证需求。技术服务实行分步报价，按节点收费，为客户节约成本。
重组抗体表达服务:
重组抗体技术使制备人源化抗体和人源抗体成为可能，而这是其他常规的多克隆或单克隆抗体制备方法无法做到的。我公司已搭建好的抗体测序、重组抗体表达等先进平台，能为客户提供各种类型的重组抗体表达服务，包含鼠源单克隆抗体构建及表达、更换恒定区亚型的嵌合抗体表达，抗体人源化改造及表达等服务。
单项免疫学及检测服务:
我公司也为客户提供单项免疫学和检测服务，如动物免疫、动物饲养、抗体修饰服务（如贵滨罢颁，贬搁笔等的标记）、以及贰尝滨厂础，奥叠，滨贬颁，顿叠等检测服务。
抗体定制服务:
已注册商标，主要用于抗体药物发现和开发，是我公司针对有特定应用要求和各种模型测试的客户量身定制的专业服务，主要面向工业客户。
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